Detecting biomarkers in body fluids is essential for diagnosing and monitoring diseases, but traditional electrochemical biosensors often face challenges such as high costs and difficult immobilization. Molecularly imprinted polymers (MIPs) offer a robust and cost-effective alternative, with high stability and suitability for wearable sensors, especially for monitoring cortisol levels. This work focuses on optimizing the production and characterization of cortisol-specific MIPs to ensure reliable and efficient use in biosensing applications.